Recombinant human Wnt3A protein

Catalog Number	OGF007
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Introduction

Recombinant human Wnt3A protein is a high-quality recombinant protein produced for research use, supporting cell culture, organoid growth, and in vitro biological studies with reliable activity and consistency.

Background

Wnt3a is a canonical Wnt ligand that activates β-catenin–dependent signaling pathways to regulate cell proliferation, polarity, and fate determination. It is essential for stem cell maintenance, intestinal homeostasis, and organoid culture systems, where it supports long-term self-renewal and expansion of stem cells.

Product Information

Species	Human
Formulation	1x PBS pH 7.4 + 1% CHAPS, See Certificate of Analysis for details
Organoid Type	Lung Organoids; Intestinal Organoids; Gastric Organoids; Liver Organoids; Pancreatic Organoids; Esophageal Organoid; Prostate Organoid
Growth Factor	Wnt3a
GeneID	89780
Expression	HEK293
Activity	25-500 ng/mL in reporter cells
Purity	>90%
Endotoxin	<1 EU/µg
Accession Number	P56704
Molecular Mass	42 kDa reduced, 40 kDa non-reduced, monomer, glycosylated
Species Reactivity	human,mouse
Stability and Storage	Lyophilized: Store at −20 °C to −80 °C until expiry date; alternatively, stable at room temperature for up to 2 weeks Reconstituted (per CoA): Stable for up to 6 months at −20 °C to −80 °C, or up to 1 week at 10 °C
Reconstitution	Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.1 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.
Synonyms	Protein Wnt 3a, WNT3A

For research use only. Not for any other purpose.

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