A dynamic microphysiological bone marrow model for hematotoxicity testing, hematopoiesis research, hematological disease modeling, and drug response evaluation under controlled perfusion conditions.
- Overview
- Details
- Advantages
- FAQs
Overview
Bone marrow is a complex hematopoietic tissue that regulates blood cell production, immune cell development, and marrow niche homeostasis. Its function depends on coordinated interactions among hematopoietic stem and progenitor cells, stromal cells, endothelial cells, immune cells, extracellular matrix, and soluble factors.
Traditional static cultures often fail to reproduce the dynamic bone marrow microenvironment, including perfusion, niche signaling, cellular crosstalk, and long-term hematopoietic regulation. Bone Marrow-on-Chip provides a perfusable in vitro model that better supports the study of marrow function, toxicity, disease mechanisms, and therapeutic responses.
What Is Bone Marrow-on-Chip?
Bone Marrow-on-Chip is a microfluidic culture platform designed to model key features of the human bone marrow niche. It supports controlled flow, co-culture of marrow-relevant cells, repeated compound exposure, and longitudinal analysis.
This platform can support the study of:
- Hematopoietic stem and progenitor cell maintenance and differentiation
- Bone marrow niche interactions and stromal support
- Drug-induced myelosuppression and hematotoxicity
- Leukemia, myeloma, and marrow-associated cancer models
- Immune cell development and inflammatory marrow responses
- Drug efficacy, resistance, and personalized response testing
Fig. 1. Conceptual biological framework and microengineered implementation of the bone marrow-on-a-chip platform (Rosalem G S, De Assis D R R, et al., 2026).
Our Bone Marrow-on-Chip Platform
Our Bone Marrow-on-Chip platform provides a dynamic marrow niche model for hematopoiesis studies, hematotoxicity assessment, hematological cancer modeling, and drug response analysis.
Key Features
- Bone marrow-like niche: Supports interactions among hematopoietic cells, stromal cells, endothelial cells, and extracellular matrix components.
- Controlled perfusion: Enables dynamic medium flow, compound exposure, nutrient exchange, and time-course sampling.
- Flexible cell options: Compatible with CD34+ hematopoietic stem/progenitor cells, bone marrow mononuclear cells, stromal cells, endothelial cells, immune cells, and disease-derived cells.
- Customizable models: Supports normal marrow, hematotoxicity, leukemia, myeloma, inflammatory marrow, and patient-derived models.
Validation & Readouts
- Cell markers: CD34, CD38, CD45, CD71, CD235a, CD11b, CD14, CD15, CD41, CD61, and lineage-specific markers
- Functional assays: Cell viability, proliferation, apoptosis, differentiation, lineage output, and colony-forming potential
- Niche and toxicity analysis: Stromal/endothelial markers, cytokines, myelosuppression, inflammatory response, and oxidative stress
- Downstream analysis: Imaging, immunostaining, flow cytometry, ELISA, qPCR, RNA-seq, and single-cell analysis
Applications
- Hematotoxicity Testing: Evaluate drug-induced bone marrow suppression and lineage-specific toxicity.
- Hematopoiesis Research: Study stem/progenitor cell maintenance, differentiation, and marrow niche regulation.
- Hematological Cancer Modeling: Investigate leukemia, myeloma, and tumor-marrow microenvironment interactions.
- Drug Response Evaluation: Assess therapeutic efficacy, resistance mechanisms, and combination treatments.
- Personalized Marrow Models: Use donor- or patient-derived cells for individualized disease modeling and drug testing.
Why Choose Our Bone Marrow-on-Chip
- More physiologically relevant than conventional static marrow cell culture
- Dynamic perfusion for controlled dosing, nutrient exchange, and longitudinal sampling
- Supports key marrow biology including hematopoiesis, niche signaling, myelosuppression, and disease modeling
- Flexible and customizable for different cell sources, co-culture designs, disease models, and assay endpoints
- Compatible with multiple readouts such as imaging, flow cytometry, biomarker assays, qPCR, RNA-seq, and single-cell analysis
- Technical support available for chip setup, model optimization, assay design, and customized workflows
FAQs
Q: What cell types can be used in Bone Marrow-on-Chip?
The platform can support CD34+ hematopoietic stem/progenitor cells, bone marrow mononuclear cells, stromal cells, endothelial cells, immune cells, cancer cells, and donor- or patient-derived marrow cells.
Q: Can this platform be used for hematotoxicity testing?
Yes. It can be used to assess myelosuppression, altered differentiation, cell viability, apoptosis, inflammatory responses, and lineage-specific toxicity.
Q: Does the chip support dynamic perfusion?
Yes. The chip can be connected to a perfusion system for controlled flow, compound exposure, medium exchange, and time-course sampling.
Q: Can it be used for leukemia or myeloma research?
Yes. The platform can be configured to study hematological malignancies, marrow niche interactions, drug sensitivity, and resistance mechanisms.
Q: What assays are compatible with this platform?
Compatible assays include imaging, immunostaining, flow cytometry, viability assays, cytokine analysis, qPCR, RNA-seq, single-cell analysis, and colony-forming assays when applicable.
Q: Do you provide customized solutions?
Yes. We can customize chip configuration, cell selection, co-culture setup, perfusion conditions, drug exposure protocols, and endpoint assays.
Advance hematotoxicity testing and hematological disease modeling with our human-relevant Bone Marrow-on-Chip platform.
Contact us today to request product details, pricing, or customized solutions for your study.
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