Ready-to-use 3D human liver organoids that replicate key liver functions, designed to accelerate drug discovery, toxicity testing, and disease modeling
- Overview
- Details
- Advantages
- FAQs
Overview
The human liver maintains important physiological functions such as metabolism, detoxification, protein expression, and bile production. However, existing models including 2D cell lines and animal models fail to recapitulate human-specific 3D micro-anatomy and cellular heterogeneity of the liver. This presents an unmet need for more predictive models in drug development and liver research.
Liver organoids are emerging as advanced in vitro models for hepatology research. Derived from human induced pluripotent stem cells (hiPSCs), organoids can self-assemble into highly organized 3D tissue that mimics key aspects of the in vivo microenvironment, organization, and functionality of hepatocytes and cholangiocytes. They serve as a human platform that reduces species-related differences associated with animal models and limitations associated with primary hepatocytes, such as donor variability and limited stability in culture.
What Are Liver Organoids?
Organoids are miniature, self-assembled, multicellular structures derived from stem cells that recapitulate key structural and functional features of their corresponding organs. Liver organoids specifically resemble key attributes of the human liver, including:
- 3D multicellular organization
- Functional hepatocytes and biliary cells
- Key liver-specific metabolic and secretory activities
- Extended culture capability under defined conditions
Liver organoids can be generated from tissue, progenitor cells, or stem cells and can be cultured long-term in defined 3D media that promote differentiation and polarization.
Fig. 1. Schematic representation of the generation of liver organoids (Liu S, Cheng C, et al., 2024).
Our Ready-to-Use Liver Organoids
Our liver organoids are high-quality, ready-to-use human liver models. We develop organoids from human induced pluripotent stem cells with a precision-controlled 3D environment which ensures hepatic identity and functionality while supporting reproducibility and scalability.
Key Features
- High fidelity to in vivo liver tissue. Express key hepatic markers including ALB, HNF4α, and CYP3A4
- Functionally relevant organoid with metabolic activity including secretion of urea, glycogen storage, and Cytochrome P450 activity
- Batch-to-batch consistency. Every vial goes through a rigorous quality control process to ensure uniform size and reproducible functionality
- Ready-to-use. Our organoids are cryopreserved with optimized recovery media to allow you to skip weeks of differentiation and media optimization.
Characterization & Validation
Our liver organoids are validated through molecular, structural, and functional assays to ensure biological relevance and reproducibility.
- Marker expression: ALB, HNF4α, A1AT, and CK19 confirmed by immunostaining and qPCR
- 3D morphology: Organized architecture with polarized cells and lumen-like structures
- Liver functions: Albumin secretion, urea production, glycogen storage, and active CYP450 (e.g., CYP3A4)
- Quality control: Post-thaw viability ≥85%, low batch variability, mycoplasma-free
Applications
Our ready-to-use liver organoids are a powerful platform for a wide range of applications:
- Disease Modeling: Model liver diseases such as genetic disease (Alpha-1 Antitrypsin Deficiency, Alagille Syndrome), metabolic disease (NAFLD/NASH), and viral infection (HBV, HCV).
- Drug Discovery & Toxicity Screening: Assess drug efficacy, hepatotoxicity (Drug-Induced Liver Injury or DILI), and drug metabolism in a physiologically relevant system to address failure in drug development's critical stage.
- Host-Pathogen Interactions: Hepatotropic viruses such as HBV and HCV's life cycle and screen antiviral drug candidates.
- Regenerative Medicine: Explore the potential of organoids for future cell-based therapies and liver repair.
Why Choose Our Liver Organoids
- More predictive than 2D cultures with physiologically relevant liver functions
- Ready-to-use, saving weeks of differentiation time
- Reproducible, with controlled batch consistency
- HTS-compatible, supporting scalable drug screening
- Versatile, suitable for DILI, metabolism, and disease modeling
FAQs
Q: How will I receive the organoids shipment?
Liver organoids are shipped to you in cryopreserved vials on either dry ice or in liquid nitrogen dewars. Upon arrival, they can be quickly recovered and plated in standard cell culture conditions (37°C, 5% CO2).
Q: How long will I be able to culture the organoids after thawing?
We typically recommend a culture period of 3-7 days for most assays. However, the organoids can be maintained for extended periods under optimized conditions.
Q: Can organoids be used in co-culture setups?
Yes. The 3D structure of organoids makes them ideal for co-culture with other cell types such as immune cells or fibroblasts to better mimic the in vivo liver microenvironment.
Q: Do you offer diseased or genetically engineered liver organoids?
Currently, we offer wild-type organoids derived from healthy donors. We are actively developing disease-specific models (e.g., for metabolic syndrome, genetic disorders). Please contact us for future availability.
Accelerate your liver research with physiologically relevant, ready-to-use human liver organoids.
Contact us today to request detailed datasheets, pricing information, or customized solutions tailored to your study needs.
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