Ready-to-use 3D human gastric organoids that model stomach structure and function for disease research, drug screening, and host–pathogen interaction studies
- Overview
- Details
- Advantages
- FAQs
Overview
The human stomach plays essential roles in digestion, acid secretion, and mucosal defense. Traditional in vitro models such as 2D gastric cell cultures lack the structural complexity and cellular diversity of native gastric tissue, while animal models may not fully reflect human-specific gastric physiology. These limitations create a demand for more predictive and human-relevant gastric models.
Gastric organoids, also known as human gastric organoids or stomach organoids, are advanced 3D culture systems derived from stem cells. These organoids self-organize into gland-like structures that recapitulate key features of the stomach, providing a physiologically relevant platform for gastric disease modeling, drug screening, and infection studies.
What Are Gastric Organoids?
Gastric organoids are multicellular 3D structures derived from human induced pluripotent stem cells (hiPSCs) or adult gastric stem cells. They mimic essential characteristics of gastric tissue, including:
- 3D glandular architecture resembling native gastric glands
- Multiple gastric epithelial cell types (mucous cells, chief cells, enteroendocrine cells)
- Expression of stomach-specific markers and secretory functions
- Long-term expansion and differentiation under defined culture conditions
These human gastric organoids provide a robust in vitro system for studying gastric development, epithelial homeostasis, and disease mechanisms.
Fig. 1. Generation of stomach organoids from adult stem cells (AdSCs) and pluripotent stem cells (PSCs)
(Seidlitz T, Koo B, et al., 2020).
Our Ready-to-Use Gastric Organoids
Our human gastric organoids are generated under controlled 3D culture conditions to ensure reproducibility and biological relevance. These ready-to-use gastric organoids provide a reliable platform for gastric disease models, drug discovery, and host–pathogen interaction studies.
Key Features
- Physiologically relevant gastric models expressing markers such as MUC5AC, MUC6, PGC, and TFF1
- Gland-like morphology reflecting stomach epithelial organization
- Functional properties including mucus secretion and epithelial barrier characteristics
- Cryopreserved and ready-to-use to support efficient experimental workflows
Characterization & Validation
Our gastric organoids are validated using structural and molecular assays to ensure consistency and functionality.
- Marker expression: MUC5AC, MUC6, PGC, TFF1 confirmed by immunostaining and qPCR
- 3D morphology: Glandular structures with polarized epithelial organization
- Functional readouts: Mucus production and epithelial integrity
- Quality control: Post-thaw viability ≥85%, batch consistency, mycoplasma-free
Applications
Our gastric organoids are suitable for a wide range of applications in gastrointestinal research:
- Disease Modeling: Study gastric diseases including gastric cancer, gastritis, and epithelial dysfunction
- Host–Pathogen Interaction: Investigate infections such as Helicobacter pylori in human gastric organoid systems
- Drug Screening & Toxicity: Evaluate drug efficacy and gastric toxicity in a physiologically relevant model
- Stem Cell Research: Explore gastric development, differentiation, and regeneration
Why Choose Our Gastric Organoids
- Human-relevant stomach models with physiological structure and function
- Ready-to-use format reduces experimental setup time
- Reproducible with strict quality control and batch consistency
- Versatile for gastric disease modeling, infection studies, and drug screening
- Scalable for medium- to high-throughput workflows
FAQs
Q: Do gastric organoids replicate stomach-specific functions such as mucus secretion?
Gastric organoids exhibit key epithelial features of the stomach, including mucus production and expression of gastric markers such as MUC5AC and MUC6. While they do not fully reproduce the entire gastric physiology (e.g., acid secretion at organ level), they provide a relevant model for studying epithelial barrier function and mucosal biology.
Q: Can gastric organoids be used for Helicobacter pylori infection studies?
Yes. Human gastric organoids are widely used to study Helicobacter pylori infection, enabling investigation of bacterial colonization, epithelial response, and host–pathogen interactions in a controlled human-relevant system.
Q: What types of gastric cells are present in these organoids?
Gastric organoids contain multiple epithelial cell types, including mucous cells, chief-like cells, and enteroendocrine cells. The exact composition may vary depending on the differentiation protocol and culture conditions.
Q: Are these gastric organoids suitable for drug screening and toxicity studies?
Yes. Gastric organoids provide a physiologically relevant platform for evaluating drug responses, epithelial toxicity, and compound effects on gastric barrier integrity, making them suitable for early-stage drug screening.
Q: How do gastric organoids compare to intestinal organoids?
Gastric organoids model stomach-specific epithelial biology, including glandular structure and mucus secretion, whereas intestinal organoids exhibit crypt–villus architecture and nutrient absorption functions. The choice depends on the specific research focus and target tissue.
Q: What are the recommended applications for gastric organoids?
Gastric organoids are commonly used in gastric disease modeling (e.g., gastritis, gastric cancer), H. pylori infection studies, drug screening, and research on epithelial differentiation and regeneration.
Advance your gastric research with reliable, ready-to-use human gastric organoids.
Contact us for datasheets, pricing, or customized gastric organoid solutions tailored to your research needs.
Online Inquiry