Recombinant human HGF NK1 protein

Catalog Number	OGF021
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Introduction

Recombinant human HGF NK1 protein is a recombinant growth factor designed for in vitro research, widely used in cell culture and organoid-related studies to support cell growth and functional analysis.

Background

Hepatocyte growth factor (HGF), also known as scatter factor (SF), is a multifunctional cytokine that regulates proliferation, survival, motility, angiogenesis, and tissue regeneration. HGF is synthesized as a single inactive precursor and proteolytically cleaved into an active heterodimer composed of α and β chains linked by a disulfide bond. Its biological activity is mediated through binding to the receptor tyrosine kinase c-Met, activating PI3K/AKT, ERK, FAK, and other signaling pathways.

The NK1 isoform is a naturally occurring splice variant comprising the N-terminal hairpin loop and the first kringle domain of HGF. This fragment retains receptor-binding capacity and exhibits partial agonist activity toward c-Met.

HGF and its NK1 variant are widely used in cell culture applications involving hepatocyte survival, endothelial biology, wound healing, mesenchymal cell differentiation, and regenerative medicine models. Aberrant HGF/c-Met signaling is implicated in tumor progression and metastasis.

Product Information

Species	Human
Formulation	1x PBS, See Certificate of Analysis for details
Organoid Type	Breast organoids
Growth Factor	HGF NK1
GeneID	3082
Expression	HEK293
Activity	≈1.5 ng/mL
Purity	>95%
Endotoxin	<1 EU/µg
Accession Number	P14210
Molecular Mass	~34–38 kDa
Species Reactivity	human species similarity: mouse - 89% rat - 90% bovine - 93% porcine - 95%
Stability and Storage	Lyophilized: Store at −20 °C to −80 °C until expiry date; alternatively, stable at room temperature for up to 2 weeks Reconstituted (per CoA): Stable for up to 6 months at −20 °C to −80 °C, or up to 1 week at 24 °C
Reconstitution	Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.6% endotoxin-free recombinant serum albumin (HSA). Gently swirl or tap vial to mix.
Synonyms	Hepatopoietin-A, Scatter factor, SF, HGF-NK1, HGF (NK1), Qk13

For research use only. Not for any other purpose.

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