Recombinant human FGF-8b protein

Catalog Number OGF023
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Introduction

Recombinant human FGF-8b protein is a recombinant growth factor designed for in vitro research, widely used in cell culture and organoid-related studies to support cell growth and functional analysis.

Background

FGF-8b is a splice variant of fibroblast growth factor 8 (FGF-8), a member of the FGF family of heparin-binding growth factors. Human FGF-8 generates multiple isoforms through alternative splicing, with FGF-8b exhibiting high receptor affinity and strong biological potency.

FGF-8b binds and activates fibroblast growth factor receptors (FGFRs), particularly FGFR1c and FGFR3c isoforms, initiating Ras/MAPK and PI3K signaling pathways. It plays a critical role during embryogenesis, especially in gastrulation, limb development, midbrain patterning, and epithelial-mesenchymal signaling interactions.

In vitro, recombinant FGF-8b is widely used in neuronal differentiation protocols, dopaminergic neuron generation, midbrain organoid formation, and neural regeneration studies.

Product Information

Species Human
Formulation 1x PBS, See Certificate of Analysis for details
Organoid Type Brain organoids
Growth Factor FGF-8b
GeneID 2265
Expression HEK293
Activity 0.7-60 ng/mL
Purity >95%
Endotoxin <1 EU/µg
Accession Number P22455
Molecular Mass ~22.5 kDa
Species Reactivity human species similarity: mouse - 100%
Stability and Storage
  • Lyophilized: Store at −20 °C to −80 °C until expiry date; alternatively, stable at room temperature for up to 2 weeks
  • Reconstituted (per CoA): Stable for up to 6 months at −20 °C to −80 °C, or up to 1 week at 26 °C
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.8% endotoxin-free recombinant serum albumin (HSA). Gently swirl or tap vial to mix.
Synonyms AIGF, AIGFKAL6, Androgen-induced growth factor, FGF8, FGF-8, Fibroblast Growth Factor – 8, HBGF-8, Heparin Binding Growth Factor – 8, MGC149376, FGF8b, FGF 8b, Qk57
For research use only. Not for any other purpose.

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