Recombinant human FGF-10 protein

Catalog Number OGF025
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Introduction

Recombinant human FGF-10 protein is a recombinant growth factor designed for in vitro research, widely used in cell culture and organoid-related studies to support cell growth and functional analysis.

Background

FGF-10 is a mesenchyme-derived member of the FGF family and is highly similar to FGF-7. It primarily signals through FGFR2b expressed on epithelial cells, mediating epithelial-mesenchymal interactions during organogenesis.

FGF-10 plays crucial roles in limb bud formation, lung branching morphogenesis, and development of multiple organs including heart, liver, kidney, thymus, mammary gland, and pancreas. It establishes epithelial-mesenchymal signaling feedback loops essential for organ patterning.

In stem cell and organoid systems, recombinant FGF-10 promotes epithelial differentiation, enhances organoid growth and branching, and supports differentiation toward gut, lung, and hepatic lineages. Dysregulated FGF-10 signaling has been associated with developmental disorders and certain cancers.

Product Information

Species Human
Formulation 1x PBS, See Certificate of Analysis for details
Organoid Type Lung organoids; Breast organoids
Growth Factor FGF-10
GeneID 2255
Expression HEK293
Activity ≈0.36 ng/mL
Specific Activity ≥ 1.00 x 10^6 IU/mg
Purity >95%
Endotoxin <1 EU/µg
Accession Number P57774
Molecular Mass ~19 kDa
Species Reactivity human species similarity: mouse - 94% rat - 100% bovine - 99% porcine - 99%
Stability and Storage
  • Lyophilized: Store at −20 °C to −80 °C until expiry date; alternatively, stable at room temperature for up to 2 weeks
  • Reconstituted (per CoA): Stable for up to 6 months at −20 °C to −80 °C, or up to 1 week at 28 °C
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.10% endotoxin-free recombinant serum albumin (HSA). Gently swirl or tap vial to mix.
Synonyms Fibroblast Growth Factor 10, FGFA, KGF2, Keratinocyte growth factor 2, FGF10, FGF 10, Qk3, Qk03
For research use only. Not for any other purpose.

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