Branching Lung Organoid Kit

Catalog Number OCM006
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Introduction

A serum-free system for generating branching lung organoids from human pluripotent stem cells through a four-stage differentiation process encompassing definitive endoderm, anterior foregut endoderm, lung bud organoid, and branching lung organoid stages. The resulting organoids exhibit proximal and distal-like branching airway epithelial structures expressing EPCAM, NKX2.1, SOX2, SOX9, MUC1, and P63. Extended culture beyond 28 days in matrix sandwich configurations shows increased expression of mature lung cell markers. Optimized for cells maintained in standard pluripotent stem cell media, with maturation components available separately for long-term culture.

Application

Differentiation, Genome Editing, Organoid Culture

Feature

Recapitulates key morphogenetic events of lung branching, providing a dynamic model to study pulmonary development and regenerative medicine.

Product Information

Species Human
Formulation Serum-Free
Cell Type Epithelial Cells, PSC-Derived, Mesenchymal Cells, PSC-Derived, Pluripotent Stem Cells
For research use only. Not for any other purpose.

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