Apical-Out Airway Organoid Secretory Medium

Catalog Number OCM023
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Introduction

A system for generating mature apical-out airway organoids containing basal, ciliated, and secretory cells in 15 days from human primary airway epithelial cells using a simple scalable matrix-free protocol. Producing polarized organoids with outward-facing architecture providing direct apical surface access for infectious disease modeling and high-throughput drug screening in three dimensions, this medium includes a specialized supplement to promote secretory cell development and better reflect cellular diversity of human airway epithelium. Compatible with specialized microwell plate culture without cell culture inserts, the serum-free and bovine pituitary extract-free formulation ensures experimental consistency. For optimal results, expand cells in compatible growth medium prior to differentiation.

Application

Cell Culture, Differentiation, Organoid Culture

Feature

The apical-out geometry naturally exposes the luminal surface, dramatically simplifying infection protocols and quantitative drug uptake measurements.

Product Information

Species Human
Formulation Serum-Free
Cell Type Airway Cells, Epithelial Cells
For research use only. Not for any other purpose.

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